Detailed view for EHI_189850

Basic information

TDR Targets ID: 797750
Entamoeba histolytica, V-type ATPase, B subunit, putative
Source Database / ID:  AmoebaDB  

pI: 4.7253 | Length (AA): 496 | MW (Da): 55344 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00006   ATP synthase alpha/beta family, nucleotide-binding domain
PF02874   ATP synthase alpha/beta family, beta-barrel domain

Gene Ontology

Mouse over links to read term descriptions.
GO:1902600   GO:proton transmembrane transport  

GO:0033180   proton-transporting V-type ATPase, V1 domain  
GO:0005524   ATP binding  
GO:0046034   ATP metabolic process  
GO:0015991   ATP hydrolysis coupled proton transport  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)
Phagosome (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 493 5dn6 (A) 3 486 22.00 0 1 1.26682 -0.26
22 480 3vr4 (D) 4 453 58.00 0 1 1.7101 -1.31
22 482 5d80 (D) 27 486 77.00 0 1 1.84414 -0.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127061)

Species Accession Gene Product
Arabidopsis thaliana AT1G20260   V-type proton ATPase subunit B3
Arabidopsis thaliana AT1G76030   V-type proton ATPase subunit B1
Arabidopsis thaliana AT4G38510   V-type proton ATPase subunit B2
Babesia bovis BBOV_II001490   vacuolar ATPase subunit B
Brugia malayi Bm1_12010   vacuolar ATP synthase subunit B
Candida albicans CaO19.6634   vacuolar ATPase V1 domain subunit B
Candida albicans CaO19.13955   vacuolar ATPase V1 domain subunit B
Caenorhabditis elegans CELE_Y110A7A.12   Protein SPE-5
Caenorhabditis elegans CELE_F20B6.2   Protein VHA-12
Cryptosporidium hominis Chro.80195   vacuolar ATP synthase subunit b
Cryptosporidium parvum cgd8_1670   vacuolar ATP synthase subunit B
Chlamydia trachomatis CT_307   V-type ATP synthase subunit B
Dictyostelium discoideum DDB_G0277401   vacuolar H+ ATPase B subunit
Drosophila melanogaster Dmel_CG17369   Vacuolar H[+]-ATPase 55kD subunit
Echinococcus granulosus EgrG_001077900   vacuolar ATP synthase subunit b
Entamoeba histolytica EHI_189850   V-type ATPase, B subunit, putative
Echinococcus multilocularis EmuJ_001077900   vacuolar ATP synthase subunit b
Giardia lamblia GL50803_12216   Vacuolar ATP synthase subunit B
Homo sapiens ENSG00000116039   ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Homo sapiens ENSG00000147416   ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Leishmania braziliensis LbrM.28.2630   vacuolar ATP synthase subunit b, putative
Leishmania donovani LdBPK_282610.1   Vacuolar proton pump subunit B, putative
Leishmania infantum LinJ.28.2610   vacuolar ATP synthase subunit b, putative
Leishmania major LmjF.28.2430   vacuolar ATP synthase subunit b, putative
Leishmania mexicana LmxM.28.2430   vacuolar ATP synthase subunit b, putative
Loa Loa (eye worm) LOAG_07342   vacuolar ATP synthase subunit B
Mus musculus ENSMUSG00000006269   ATPase, H+ transporting, lysosomal V1 subunit B1
Mus musculus ENSMUSG00000006273   ATPase, H+ transporting, lysosomal V1 subunit B2
Neospora caninum NCLIV_060820   V-type ATP synthase beta chain, related
Oryza sativa 4341339   Os06g0568200
Oryza sativa 4327833   Os01g0711000
Plasmodium berghei PBANKA_1003800   V-type proton ATPase subunit B, putative
Plasmodium falciparum PF3D7_0406100   V-type proton ATPase subunit B
Plasmodium knowlesi PKNH_0304200   V-type proton ATPase subunit B, putative
Plasmodium vivax PVX_000935   vacuolar ATP synthase subunit b, putative
Plasmodium yoelii PY05102   V-type ATPase, B subunit
Saccharomyces cerevisiae YBR127C   H(+)-transporting V1 sector ATPase subunit B
Schistosoma japonicum Sjp_0031510   ko:K02147 V-type H+-transporting ATPase subunit B, putative
Schistosoma japonicum Sjp_0116110   Probable vacuolar ATP synthase subunit B, putative
Schistosoma mansoni Smp_029390   ATP synthase subunit beta vacuolar
Schmidtea mediterranea mk4.001230.01   V-type proton ATPase subunit B
Trypanosoma brucei gambiense Tbg972.11.13110   vacuolar ATP synthase subunit b, putative,v- ATPase B subunit,vacuolar proton pump B subunit
Trypanosoma brucei Tb927.11.11690   Vacuolar proton pump subunit B, putative
Trypanosoma congolense TcIL3000.11.12320   Vacuolar proton pump subunit B, putative
Trypanosoma cruzi TcCLB.511209.10   Vacuolar proton pump subunit B, putative
Trypanosoma cruzi TcCLB.506025.50   V-type proton ATPase subunit B, putative
Toxoplasma gondii TGME49_219800   vacuolar ATP synthase subunit b, putative
Treponema pallidum TP0528   V-type ATP synthase subunit B
Treponema pallidum TP0427   V-type ATP synthase subunit B
Theileria parva TP04_0441   vacuolar ATP synthase subunit B, putative
Trichomonas vaginalis TVAG_453110   ATP synthase alpha subunit mitochondrial, putative
Trichomonas vaginalis TVAG_324980   ATP synthase, putative

Essentiality

EHI_189850 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.3560 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.3560 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.3560 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.3560 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F20B6.2 Caenorhabditis elegans adult lethal wormbase
CELE_F20B6.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_F20B6.2 Caenorhabditis elegans larval arrest wormbase
CELE_F20B6.2 Caenorhabditis elegans larval lethal wormbase
CELE_F20B6.2 Caenorhabditis elegans slow growth wormbase
CELE_F20B6.2 Caenorhabditis elegans sterile wormbase
PBANKA_1003800 Plasmodium berghei Essential plasmo
TGME49_219800 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 Compounds References
Gallus gallus Adenosinetriphosphatase Compounds References
Homo sapiens ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Mycobacterium tuberculosis Probable ATP synthase beta chain AtpD 486 aa 26.4% 431 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0165 1 1
0.0177 1 1
0.017 0.3424 1
0.0194 1 1
0.0044 1 1
0.0213 1 1
0.017 0.3424 1
0.0094 1 1
0.0194 1 1
0.0174 1 1
0.018 1 1
0.0043 1 1
0.0191 1 1
0.0191 1 1
0.0054 1 1
0.0044 1 1
0.0192 0.4736 1
0.0192 0.4736 1
0.0212 1 1
0.0177 1 1
0.0092 0.3006 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_189850 (Entamoeba histolytica), V-type ATPase, B subunit, putative
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